아지노모도㈜의 아미노산 기술과 30년이상 동물세포배양배지사업에 대한 경험을 바탕으로 개발된 동물세포배양배지입니다.
최근엔 모두 Chemically Defined 성분으로 동물유래성분을 포함하지 않은 고성능 배지를 제조·판매하고 있습니다.
By using media supplements for cell culture experiments, there are many advantages for titer improvement as well as cost effectiveness. CELLiST™ AminoSupplement help ensure the reliability and consistency of your cell culture and research results.
| Product name | Main Target | Format | Package type | Qty. |
|---|---|---|---|---|
| AminoSupplement Cys1 | Single-agent feed media users | Powder | 1 L Aluminum pouch | 6.61 g |
| 50 L Plastic Drum | 331 g | |||
| AminoSupplement Cys2 | Two-agent feed media users | Powder | 1 L Aluminum pouch | 17.0 g |
| 50 L Plastic Drum | 425 g |
Adding AminoSupplement Cys1/2 increase Cysteine availability in the media by inhibiting Cystine formation. This leads to improvement in overall productivity, as can be seen below.
Fed-batch cultures were compared using cells of CHO-DG44. FEED2 was added 5.25% on days 4, 6, 8, 10, and 12 of culture.
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 6% to the basic media from other companies on culture days 4, 6, 8, 10, and 12.
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 4.5% to the basic media from other companies on cultures 4, 6, 8, 10, and 12 days.
Adding AminoSupplement Cys1/Cys2 has no major impact on glycan profile and Charge Variant as follows, so you can use it without any significant change in the culture process.
Two-agent feed media requires two tanks due to instability. However, adding AminoSupplement Cys2 improves the stability, which allows it to be prepared in a single tank and save much initial & running costs.
| day | 0 | 10 | 14 | 17 | 21 | 24 | 28 | 31 | |
| Other feed media(feed A+B) w/o AminoSupplement Cys2 |
10 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
|---|---|---|---|---|---|---|---|---|---|
| 40 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| Other feed media(feed A) w/ AminoSupplement Cys2 |
10 mL | — | — | — | — | — | — | — | + |
| 40 mL | — | — | — | — | — | — | — | — |
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